A Taste Bud Organoid-Based Microelectrode Array Biosensor for Taste Sensing

The biological taste system has the unique ability to detect substances. Biomaterials originating from a have been recognized as ideal candidates serve sensitive elements in development of taste-based biosensors. In this study, we developed bud organoid-based biosensor for research sensation. Taste organoids prepared newborn mice were cultured and loaded onto surface 64-channel microelectrode array (MEA) chip explore electrophysiological changes upon taste; an MEA was used simultaneously record multiple-neuron firing activities under different stimuli, which helped reveal role buds sensing. obtained results show that cells separated epithelium grew well into spherical structures 3D culture conditions. These composed multiple with obvious budding structures. Moreover, multicellular spheres seeded on processed stimuli. It indicated could efficiently monitor signals response various This provides new method study sensations functions.